کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9038804 | 1133873 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Epidermal-skin-test 1000 (EST-1000)-A new reconstructed epidermis for in vitro skin corrosivity testing
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کلمات کلیدی
ET503-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide - 3- [4،5-dimethylthiazol-2-yl] -2،5-diphenyltetrazolium bromideMTT - MTTIn vitro testing - تست آزمایشگاهیSkin corrosion - خوردگی پوستAlternative method - روش جایگزینcytokeratin - سیتوکراتینSDS, sodium dodecyl sulfate - ﺳﺪﯾﻢ دودﺳﯿﻞ ﺳﻮﻟﻔﺎت
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
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چکیده انگلیسی
The determination of a possible corrosive or irritative potential of certain products and ingredients is necessary for their classification and labeling requirements. Reconstructed skin as a model system provides fundamental advantages to single cell culture testing and leads to promising results as shown by different validation studies (for review: Fentem, J.H., Botham, P.A., 2002. ECVAM's activities in validating alternative tests for skin corrosion and irritation. ATLA 30(Suppl. 2), 61-67). In this study we introduce our new reconstructed epidermis “Epidermal-Skin-Test” (EST-1000). This fully grown epidermis consists of proliferating as well as differentiating keratinocytes. EST-1000 shows a high comparability to normal human skin as shown by histological and immunohistochemical data. Characteristic markers (KI-67, CK 1/10/5/14, transglutaminase, collagen IV, involucrin, beta 1 integrin) can be identified easily. The main focus of this work was to characterize EST-1000 especially with respect to its barrier function by testing several substances of known corrosive potential. Skin corrosion was detected by the cytotoxic effect of the substances on a reconstructed epidermis after short-term application to the stratum corneum. The effect was determined by standard MTT assay and accompanying histological analysis. Hence EST-1000 shows a very high predictive potential and closes the gap between animal testing and the established full-thickness model Advanced-Skin-Test 2000 (AST-2000) (Noll, M., Merkle, M.-L., Kandsberger, M., Matthes, T., Fuchs, H., Graeve, T., 1999. Reconstructed human skin (AST-2000) as a tool for pharmaco-toxicology. ATLA 27, 302).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 19, Issue 7, October 2005, Pages 925-929
Journal: Toxicology in Vitro - Volume 19, Issue 7, October 2005, Pages 925-929
نویسندگان
J. Hoffmann, E. Heisler, S. Karpinski, J. Losse, D. Thomas, W. Siefken, H.-J. Ahr, H.-W. Vohr, H.W. Fuchs,