کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9131869 | 1160958 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection of potential GDF6 regulatory elements by multispecies sequence comparisons and identification of a skeletal joint enhancer
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Detection of potential GDF6 regulatory elements by multispecies sequence comparisons and identification of a skeletal joint enhancer Detection of potential GDF6 regulatory elements by multispecies sequence comparisons and identification of a skeletal joint enhancer](/preview/png/9131869.png)
چکیده انگلیسی
The identification of noncoding functional elements within vertebrate genomes, such as those that regulate gene expression, is a major challenge. Comparisons of orthologous sequences from multiple species are effective at detecting highly conserved regions and can reveal potential regulatory sequences. The GDF6 gene controls developmental patterning of skeletal joints and is associated with numerous, distant cis-acting regulatory elements. Using sequence data from 14 vertebrate species, we performed novel multispecies comparative analyses to detect highly conserved sequences flanking GDF6. The complementary tools WebMCS and ExactPlus identified a series of multispecies conserved sequences (MCSs). Of particular interest are MCSs within noncoding regions previously shown to contain GDF6 regulatory elements. A previously reported conserved sequence at â64 kb was also detected by both WebMCS and ExactPlus. Analysis of LacZ-reporter transgenic mice revealed that a 440-bp segment from this region contains an enhancer for Gdf6 expression in developing proximal limb joints. Several other MCSs represent candidate GDF6 regulatory elements; many of these are not conserved in fish or frog, but are strongly conserved in mammals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Genomics - Volume 86, Issue 3, September 2005, Pages 295-305
Journal: Genomics - Volume 86, Issue 3, September 2005, Pages 295-305
نویسندگان
Matthew E. Portnoy, Kelly J. McDermott, Anthony Antonellis, Elliott H. Margulies, Arjun B. Prasad, NISC Comparative Sequencing Program NISC Comparative Sequencing Program, David M. Kingsley, Eric D. Green, Douglas P. Mortlock,