کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9131978 | 1160973 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization and expression analysis of the Spink5 gene, the mouse ortholog of the defective gene in Netherton syndrome
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Characterization and expression analysis of the Spink5 gene, the mouse ortholog of the defective gene in Netherton syndrome Characterization and expression analysis of the Spink5 gene, the mouse ortholog of the defective gene in Netherton syndrome](/preview/png/9131978.png)
چکیده انگلیسی
The human SPINK5 gene, encoding the putative 15-domain serine protease inhibitor LEKTI, was identified as the defective gene in the severe autosomal recessive ichthyosiform skin disorder known as Netherton syndrome and as a candidate susceptibility gene for atopic disease. Here we report mapping of the murine Spink5 gene to chromosome 18 and its characterization. We show that, unlike in humans, transcription of the mouse Spink5 gene generates two mRNAs that differ in the 3â² untranslated region. The encoded protein, which is detected in differentiated primary cultured keratinocytes and mouse skin as an â¼130-kDa glycosylated precursor, displays â¼60% identity with its human counterpart but lacks the human LEKTI domain 6. As in the human, mouse Lekti represents a marker of epithelial differentiation, strongly expressed in the granular layer of the epidermis, in suprabasal layers of stratified epithelia, and in thymic Hassall's bodies. Our data indicate that mouse Spink5/Lekti, like its human counterpart, is involved in the control of epithelial tissue homeostasis, but also highlight specific features of the murine gene and protein.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Genomics - Volume 85, Issue 4, April 2005, Pages 483-492
Journal: Genomics - Volume 85, Issue 4, April 2005, Pages 483-492
نویسندگان
Marie Florence Galliano, Rosa Maria Roccasecca, Pascal Descargues, Alessia Micheloni, Elaine Levy, Giovanna Zambruno, Marina D'Alessio, Alain Hovnanian,