کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9131988 | 1160975 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mouse protein arrays from a TH1 cell cDNA library for antibody screening and serum profiling
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Mouse protein arrays from a TH1 cell cDNA library for antibody screening and serum profiling Mouse protein arrays from a TH1 cell cDNA library for antibody screening and serum profiling](/preview/png/9131988.png)
چکیده انگلیسی
The mouse is the premier genetic model organism for the study of disease and development. We describe the establishment of a mouse T helper cell type 1 (TH1) protein expression library that provides direct access to thousands of recombinant mouse proteins, in particular those associated with immune responses. The advantage of a system based on the combination of large cDNA expression libraries with microarray technology is the direct connection of the DNA sequence information from a particular clone to its recombinant, expressed protein. We have generated a mouse TH1 expression cDNA library and used protein arrays of this library to characterize the specificity and cross-reactivity of antibodies. Additionally, we have profiled the autoantibody repertoire in serum of a mouse model for systemic lupus erythematosus on these protein arrays and validated the putative autoantigens on highly sensitive protein microarrays.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Genomics - Volume 85, Issue 3, March 2005, Pages 285-296
Journal: Genomics - Volume 85, Issue 3, March 2005, Pages 285-296
نویسندگان
Claudia Gutjahr, Derek Murphy, Angelika Lueking, Andrea Koenig, Michal Janitz, John O'Brien, Bernhard Korn, Sabine Horn, Hans Lehrach, Dolores J. Cahill,