کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9191881 | 1186603 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Glutamate promotes NF-κB pathway in primary astrocytes: protective effects of IRFI 016, a synthetic vitamin E analogue
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کلمات کلیدی
NF-κBDcf2′-7′-dichlorofluoresceinGYKI 524663-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyl tetrazolium bromideGSH/GSSG - GSH / GSSGMTT - MTTROS - ROSPrimary astrocytes - آستروسیت های اولیهROS production - تولید ROSlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH Glutathione - گلوتاتیونreduced/oxidized glutathione - گلوتاتیون کاهش / اکسید شدهglutamate - گلوتاماتReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
عصب شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Oxidative stress has been implicated in several neurodegenerative diseases affecting both neuronal and glial cells. The aim of this study was to investigate the involvement of reactive oxygen species in glutamate-evoked activation of NF-κB in primary astrocytes. A prolonged exposure to glutamate (24 h) caused a depletion of intracellular glutathione that, in astroglial cells, has been considered a biochemical change typical of early astrocyte dysfunction, leading to cell alterations occurring in the gliosis. These effects were initiated by AMPA/KA receptor activation and almost completely blocked by anti-oxidants. Indeed, we provide evidence that the incubation of primary astrocytes with a hydrophilic derivative of tocopherol, such as IRFI 016, was useful to reduce glutamate-induced oxidative effects. This agent also reduced in a dose-dependent manner the nuclear translocation of both p50 and p65 subunits of NF-κB. Altogether, these data confirm that GSH content plays a pivotal role to determine oxidative response to glutamate injury in primary astrocyte cultures and that NF-κB pathway is involved in this response. Furthermore, the positive effects obtained by IRFI 016 to prevent nuclear translocation of NF-κB may suggest new pharmacological strategies for antioxidant therapy and neuroprotection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Neurology - Volume 193, Issue 2, June 2005, Pages 377-383
Journal: Experimental Neurology - Volume 193, Issue 2, June 2005, Pages 377-383
نویسندگان
Daniela Caccamo, Agata Campisi, Herbert Marini, Elena B. Adamo, Giovanni Li Volti, Francesco Squadrito, Riccardo Ientile,