کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9219951 | 1199893 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Streptococcus mutans sortase catalyzes cell wall anchoring of WapA and FruA
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
دندانپزشکی، جراحی دهان و پزشکی
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چکیده انگلیسی
Previous studies using a sortase-deficient mutant (SrtAâ mutant-) of Streptococcus mutans have demonstrated that the sortase (SrtA) catalyzes cell wall anchoring of the surface protein antigen PAc, a dextranase and a glucan-binding protein C. In this study, cell wall anchoring of a wall-associated protein antigen A (WapA) and an exo-β-D-fructosidase (FruA) in S. mutans was examined by Western blot analysis with a specific antiserum. In the SrtAâ mutant, FruA and WapA were not bound to the cell wall but were secreted into the culture supernatant. In contrast, in the wild type, both proteins were associated with the cell wall of S. mutans. Biological properties of the SrtAâ mutant were examined by determination of fructan fermentation and adherence to a smooth surface. Both the SrtAâ mutant and the wild type retained the ability to ferment levan. In addition, adherence to a smooth surface of the SrtAâ mutant was as extensive as that of the wild-type 109c when sucrose was present. However, in the absence of sucrose, the adhesion of the SrtAâ mutant remarkably decreased as compared with that of the wild type. These results suggest that SrtA catalyzes anchoring of WapA and FruA to the cell wall in S. mutans and that surface proteins anchored by SrtA are involved in the initial adhesion of S. mutans to smooth surface. In addition, it was shown that both cell wall-anchored and extracellular FruA are related to the degradation of extracellular fructan as a nutrient source.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pediatric Dental Journal - Volume 15, Issue 1, 2005, Pages 127-133
Journal: Pediatric Dental Journal - Volume 15, Issue 1, 2005, Pages 127-133
نویسندگان
Chieko Murai, Mitsuko Inoue, Ryuji Sasa, Takeshi Igarashi,