کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9236523 | 1207469 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of GRASP-1 as a novel 97 kDa autoantigen localized to endosomes
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کلمات کلیدی
autoantibodiesHRSEEA1early endosome antigen 1 - آنتیژن آندوسوم اولیه 1enzyme linked immunosorbent assay - آنزیم تست ایمونوسیورسانس مرتبط استEndosomes - اندوسومELISA - تست الیزاautoimmunity - خودایمنیMass spectroscopy - طیف سنجی جرمیCytoplasmic linker protein - پروتئین لینکر سیتوپلاسمیکCanca - کانکاClip - کلیپCoiled-coils - کویل کویل
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Identification of GRASP-1 as a novel 97 kDa autoantigen localized to endosomes Identification of GRASP-1 as a novel 97 kDa autoantigen localized to endosomes](/preview/png/9236523.png)
چکیده انگلیسی
We have identified an autoantigen that is recognized by antibodies from an 18-year-old female with a history of recurrent infections who later in her clinical course developed Raynaud's phenomenon and telangiectasias. By indirect immunofluorescence (IIF), the index serum produced a unique cytoplasmic discrete speckled (CDS) staining pattern that partially colocalized with early endosome antigen 1 (EEA1) but not Golgi complex or other cytoplasmic organelles in HEp-2 cells. When HEp-2 cells were treated with 0.1 N HCl, the cytoplasmic speckled staining of the index serum was markedly decreased, suggesting that the reactive antigen was soluble. Western blot analysis showed a reactive â¼97 kDa protein in a saline soluble protein preparation from HeLa cells. Mass spectrometric analysis of the excised 97 kDa band that was immunoprecipitated from HeLa cell extracts identified GRASP-1 as a possible target. The index serum and anti-GRASP-1 antibodies colocalized to structures in the cytoplasm of HEp-2 cells. Synthetic peptides representing the full-length GRASP-1 protein were used to identify reactive epitopes. Like many other cytoplasmic autoantigens, GRASP-1 has numerous coiled-coil domains throughout the protein with the exception of short segments at the amino and carboxyl terminus.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Immunology - Volume 116, Issue 2, August 2005, Pages 108-117
Journal: Clinical Immunology - Volume 116, Issue 2, August 2005, Pages 108-117
نویسندگان
Laura M. Stinton, Sanja Selak, Marvin J. Fritzler,