کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
923762 | 921118 | 2007 | 7 صفحه PDF | دانلود رایگان |
IntroductionExercise has been shown to lower blood natural killer cell concentration and activity for up to 24-h after exercise; however, the mechanism underlying this effect is not clearly defined. We have speculated that an exercise-induced change in T-cell control of NK cells is at least partly responsible for the post-exercise suppression. As a follow-up to our previous research, the purpose of this study was to examine the T and NK cells responses during and following moderate/high-intensity endurance exercise.MethodsEight male subjects (20 ± 1 y; VO2peak = 67.22 ± 2.79 mL·kg−1·mL−1) were recruited to complete two 1-h (75–80% VO2peak) cycling trials in a random counterbalanced order: carbohydrate (CHO) and placebo (PLA). Venous blood samples were collected before (PRE), immediately (POST), 2-h (2H), and 4-h (4H) after exercise. NK (CD3−/56+) and activated NK (CD3−/56+/69+) number were measured using flow cytometry. NK cell activity (NKCA) was determined using both a 51Cr release assay (NKCA-51) and activated NK cell number (NKCA-69). Whole blood samples were stimulated with IL-2, IFN-γ, IL-4, IL-12, and no stimulation for 12-h and then analyzed for NK cell activity using a 51Cr release assay (NKCA-51).ResultsLeukocyte counts and unstimulated NKCA were not different between CHO and PLA; however, exercise significantly increased NKCA (P < .05). Fold change in IL-2, IFN-γ, and IL-2 + IFN-γ-stimulated NKCA were significantly greater in CHO compared to PLA (P < .05). No effect of drink was found for IL-4, IL-12, and IL-4 + IL-12-stimulated NKCA.DiscussionThe fold change in IL-2-stimulated NKCA is consistant with our previous published work. The drink effect for Th1 (but not Th2) cytokines suggests they may play a more significant role in modulating NKCA following a strenuous bout of aerobic exercise when CHO is consumed.
Journal: Brain, Behavior, and Immunity - Volume 21, Issue 2, February 2007, Pages 202–208