کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9266215 | 1591585 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Targeting antigens to murine and human M-cells with Aleuria aurantia lectin-functionalized microparticles
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Targeting antigens to murine and human M-cells with Aleuria aurantia lectin-functionalized microparticles Targeting antigens to murine and human M-cells with Aleuria aurantia lectin-functionalized microparticles](/preview/png/9266215.png)
چکیده انگلیسی
Neuraminidases act as a virulence factors for several pathogens that invade the human body through Peyer's patch M-cells. Because of the structural similarity of Aleuria aurantia lectin (AAL) to neuraminidases, we hypothesized that AAL might also target human M-cells. In an in vitro human M-cell co-culture model significantly more particles were transported across the epithelium when microparticles were functionalized with AAL versus those that were not. Moreover, high concentrations of AAL induced no detectable cytotoxic effects on the related intestinal epithelial cell cultures, epithelial Caco2- and HT29-MTX-E12-cells. Upon incubation with AAL, PBMCs of allergic volunteers proliferated in response to AAL and secreted the cytokines, IL-2, IFN-γ, IL-10 and IL-5 in a concentration-dependent manner, indicating immune-stimulatory properties of the lectin. We conclude that AAL-coated microparticles may have the potential to target entrapped antigens to human M-cells for oral vaccination.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunology Letters - Volume 100, Issue 2, 15 September 2005, Pages 182-188
Journal: Immunology Letters - Volume 100, Issue 2, 15 September 2005, Pages 182-188
نویسندگان
Franziska Roth-Walter, Barbara Bohle, Isabella Schöll, Eva Untersmayr, Otto Scheiner, George Boltz-Nitulescu, Franz Gabor, David J. Brayden, Erika Jensen-Jarolim,