کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9266318 1217278 2005 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The synergistic effect of phytohemagglutinin and interferon-γ on the expression of tumor necrosis factor-α from RAW 264.7 cells
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
The synergistic effect of phytohemagglutinin and interferon-γ on the expression of tumor necrosis factor-α from RAW 264.7 cells
چکیده انگلیسی
Tumor necrosis factor-α (TNF-α) is a major cytokine of host immune reaction by foreign agents. Phytohemagglutinin (PHA) is a dynamic contributor to mitogenic stimulation and augmentation of host immune defense. Interferon-γ (IFN-γ) induces induction of cytokines in macrophages and lymphocytes. The aim of this study was to examine the synergistic effects of PHA plus low dose IFN-γ on TNF-α mRNA production, cytosolic levels, and secretion in RAW 264.7 cells. The cells were stimulated with PHA or IFN-γ using various concentrations for various times. The effects of PHA on TNF-α expression appeared in dose- and time-dependent manners. The maximum doses of PHA and IFN-γ to produce them were 300 μg/ml PHA and 10 ng/ml IFN-γ. The optimum time of PHA for the TNF-α mRNA production and release were 6 and 7 h after stimulation, respectively, whereas the time of IFN-γ on them was achieved at 3 and 8 h. Although the TNF-α mRNA production, cytosolic levels, and secretion from the cells were slightly detected under 10 μg/ml PHA and 1 ng/ml IFN-γ, the combination of PHA (10 μg/ml) and IFN-γ (1 ng/ml) greatly increased them, indicating the synergistic effect of PHA plus low dose IFN-γ on TNF-α expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunology Letters - Volume 98, Issue 1, 15 April 2005, Pages 137-143
نویسندگان
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