کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9267891 1218811 2005 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Immunopathogenic role of TH1 cells in autoimmune diabetes: Evidence from a T1 and T2 doubly transgenic non-obese diabetic mouse model
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Immunopathogenic role of TH1 cells in autoimmune diabetes: Evidence from a T1 and T2 doubly transgenic non-obese diabetic mouse model
چکیده انگلیسی
To improve the feasibility of in vivo monitoring of autoreactive T cells in the diabetogenic process, we generated T1 and T2 doubly transgenic non-obese diabetic (NOD) mice in which transgenic human CD90 (hCD90) is simultaneously expressed on IFN-γ-producing cells or murine CD90.1 (mCD90.1) is expressed on IL-4-producing cells. These transgenic NOD mice develop diabetes with the same kinetics and incidence as wild type NOD mice, permitting the physiological characterization of CD4+hCD90+ cells, which represent TH1 cells in lymphoid organs and at the site of insulitis. CD4+hCD90+ cells had a higher capacity to secret IFN-γ than CD4+hCD90− cells in an autoantigen-specific manner. Transgenic mice treated with GAD65 plasmid were protected from autoimmune diabetes, and had a lower number of CD4+hCD90+ cells, confirming the pathogenic role of CD4+hCD90+ cells in autoimmune diabetes. To further investigate the effect of IL-12 on the development of TH1 cells in autoimmune diabetes, we crossed these doubly transgenic mice to IL-12p35-deficient NOD mice. Despite severe disturbance of diabetes in p35−/− mice, the frequency of TH1 cells in these mice was slightly lower than in wild type mice. These data support the pathological role of IL-12 in autoimmune diabetes and suggest the existence an IL-12-independent pathway of TH1 development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Autoimmunity - Volume 25, Issue 3, November 2005, Pages 181-192
نویسندگان
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