کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9268736 | 1219136 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid discrimination of rabies viruses isolated from various host species in Brazil by multiplex reverse transcription-polymerase chain reaction
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کلمات کلیدی
PBSMultiplex RT-PCRRT-PCREBLRFLPBrazil - برزیلFluorescent antibody test - تست آنتی بادی فلورسنتG protein - جی پروتئینPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریNucleoprotein - هسته پروتئینreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسEuropean bat lyssavirus - ویروس لوس آنجلس اروپاRabies virus - ویروس هاریL protein - پروتئین LN protein - پروتئین Nrestriction fragment length polymorphism - پلی مورفیسم طول قطعه قطعهneighbor-joining - پیوستن همسایهFat - چربیGenotype - ژنوتیپGlycoprotein - گلیکوپروتئین
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Rapid discrimination of rabies viruses isolated from various host species in Brazil by multiplex reverse transcription-polymerase chain reaction Rapid discrimination of rabies viruses isolated from various host species in Brazil by multiplex reverse transcription-polymerase chain reaction](/preview/png/9268736.png)
چکیده انگلیسی
Rabies is carried mainly by mammalian carnivores and vampire bats in Latin America. However, rabies virus (RV) has been isolated in recent years from not only vampire bats in rural areas but also from several non-vampire bat species in urban areas, respectively. Therefore, rapid molecular screening is necessary for efficient epidemiology of these RVs. In this study, we investigated the usefulness of multiplex reverse transcription-polymerase chain reaction (RT-PCR) for determining the origins of 54 RV isolates from various host species in Brazil. And to evaluate the multiplex RT-PCR as a potential diagnostic tool, we investigated the sensitivity of this method. In addition, we compared the results with a phylogenetic tree developed from sequences of the RV glycoprotein (G protein) gene. Multiplex RT-PCR products showed five different sizes of products, whereas the phylogenic tree showed six groups. Of these six groups, four corresponded with the four sizes of the multiplex RT-PCR products. The other two groups showed correspondance with another one size of the multiplex RT-PCR products, indicating that multiplex RT-PCR results reflected the lineage of the 54 isolates. This study also showed that this method can detect trace amounts of RNA. In conclusion, this multiplex RT-PCR method allows the rapid, specific, and simultaneous detection of RVs isolated from various host species in Brazil.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Clinical Virology - Volume 33, Issue 4, August 2005, Pages 267-273
Journal: Journal of Clinical Virology - Volume 33, Issue 4, August 2005, Pages 267-273
نویسندگان
Go Sato, Hitomi Tanabe, Youko Shoji, Takuya Itou, Fumio H. Ito, Tetsuo Sato, Takeo Sakai,