کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9271396 | 1220022 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparison of the activities of various alginates to induce TNF-α secretion in RAW264.7 cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروبیولوژی و بیوتکنولوژی کاربردی
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![عکس صفحه اول مقاله: Comparison of the activities of various alginates to induce TNF-α secretion in RAW264.7 cells Comparison of the activities of various alginates to induce TNF-α secretion in RAW264.7 cells](/preview/png/9271396.png)
چکیده انگلیسی
We compared the abilities of alginate polymers having different molecular sizes and compositions to induce the secretion of tumor necrosis factor (TNF)-α in RAW264.7 cells. The molecular sizes and α-L-guluronate/β-D-mannuronate (M/G) ratios of highly purified alginate polymers used in this study were 9000-38000 and 1.50-3.17, respectively. Among the alginates tested, I-S, which had the highest molecular weight, showed the most potent TNF-α-inducing activity. The M/G ratio also seemed to influence this activity, and, among alginates with similar molecular sizes, alginates with a higher M/G ratio tended to show higher activity. Interestingly, the enzymatic depolymerization of I-S with bacterial alginate lyase resulted in a dramatic increase in the TNF-α-inducing activity. Such an effect of enzymatic digestion was also observed in a relatively low-molecular-weight alginate (ULV-3), which originally had very low TNF-α-inducing activity. Furthermore, the inhibition profiles of the TNF-α-inducing activity of enzymatically digested I-S shown by three specific mitogen-activated protein (MAP) kinase inhibitors differed from those of intact I-S. These results suggest that the underlying mechanism of the TNF-α-inducing activity of enzymatically depolymerized alginate oligomers is not necessarily the same as that of original alginate polymer.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Infection and Chemotherapy - Volume 11, Issue 4, 2005, Pages 199-203
Journal: Journal of Infection and Chemotherapy - Volume 11, Issue 4, 2005, Pages 199-203
نویسندگان
Maki Kurachi, Takuji Nakashima, Kenichi Yamaguchi, Tatsuya Oda, Chihiro Miyajima, Yoshiko Iwamoto, Tsuyoshi Muramatsu, Takuji Nakashima,