کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9274353 | 1221502 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
PCR-RFLP of mitochondrial cytochrome oxidase subunit II and ITS2 of ribosomal DNA: Markers for the identification of members of the Anopheles culicifacies complex (Diptera: Culicidae)
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: PCR-RFLP of mitochondrial cytochrome oxidase subunit II and ITS2 of ribosomal DNA: Markers for the identification of members of the Anopheles culicifacies complex (Diptera: Culicidae) PCR-RFLP of mitochondrial cytochrome oxidase subunit II and ITS2 of ribosomal DNA: Markers for the identification of members of the Anopheles culicifacies complex (Diptera: Culicidae)](/preview/png/9274353.png)
چکیده انگلیسی
Anopheles culicifacies Giles is a complex of five sibling species, provisionally designated as species A, B, C, D and E. Species A, C, D and E are vectors of malaria in India. Species A, B, C and D can be identified by polytene chromosome examination except in areas where species B and E are sympatric. Species B and E share the same configuration of the polytene chromosomes but can be differentiated by examining the mitotic chromosomes of F1 progeny from field collection. Further, polytene chromosome examination method requires the mosquitoes to be at the semigravid stage, which limits on use of this method to a very small proportion of the population. The present study investigated whether the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method can be used to differentiate the members of this complex. Complete ITS2 region along with part of the 5.8S and 28S rDNA sequences (512Â bp) and the mitochondrial cytochrome oxidase II (530Â bp) were amplified and digested with different restriction endonucleases. The Alu I digest of the COII amplicon and Rsa I digest of the ITS2 amplicon could distinguish two categories: species A and D forming one category and species B, C and E forming another. Further, Dde I digestion of the COII amplicon could distinguish species E from species B and C within the latter category. The PCR-RFLP techniques developed in this study can be applied to areas where species A and B and species B and E are sympatric.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Tropica - Volume 95, Issue 2, August 2005, Pages 92-99
Journal: Acta Tropica - Volume 95, Issue 2, August 2005, Pages 92-99
نویسندگان
Geeta Goswami, K. Raghavendra, N. Nanda, S.K. Gakhar, Sarala K. Subbarao,