کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9278029 | 1593092 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Gamma delta T cell responses associated with the development of tuberculosis in health care workers
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
This study evaluated T cell immune responses to purified protein derivative (PPD) and Mycobacterium tuberculosis (Mtb) in health care workers who remained free of active tuberculosis (HCWs w/o TB), health care workers who went on to develop active TB (HCWs w/TB), non-health care workers who were TB free (Non-HCWs) and tuberculosis patients presenting with minimal (Min TB) or advanced (Adv TB) disease. Peripheral blood mononuclear cells (PBMC) were stimulated with Mtb and PPD and the expression of T cell activation markers CD25+ and HLA-DR+, intracellular IL-4 and IFN-γ production and cytotoxic responses were evaluated. PBMC from HCWs who developed TB showed decreased percentages of cells expressing CD8+CD25+ in comparison to HCWs who remained healthy. HCWs who developed TB showed increased γδ TCR+ cell cytotoxicity and decreased CD3+γδ TCRâ cell cytotoxicity in comparison to HCWs who remained healthy. PBMC from TB patients with advanced disease showed decreased percentages of CD25+CD4+ and CD25+CD8+ T cells that were associated with increased IL-4 production in CD8+ and γδ TCR+ phenotypes, in comparison with TB patients presenting minimal disease. TB patients with advanced disease showed increased γδ TCR+ cytotoxicity and reduced CD3+γδ TCRâ cell cytotoxicity. Our results suggest that HCWs who developed TB show an early compensatory mechanism involving an increase in lytic responses of γδ TCR+ cells which did not prevent TB.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEMS Immunology and Medical Microbiology - Volume 43, Issue 3, 1 March 2005, Pages 339-350
Journal: FEMS Immunology and Medical Microbiology - Volume 43, Issue 3, 1 March 2005, Pages 339-350
نویسندگان
Diane J. Ordway, Luisa Pinto, Leonor Costa, Marta Martins, Clara Leandro, Miguel Viveiros, Leonard Amaral, Maria J. Arroz, Fernando A. Ventura, Hazel M. Dockrell,