کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9279551 | 1593516 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression and spectroscopic analysis of a mutant hepatitis B virus onco-protein HBx without cysteine residues
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
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![عکس صفحه اول مقاله: Expression and spectroscopic analysis of a mutant hepatitis B virus onco-protein HBx without cysteine residues Expression and spectroscopic analysis of a mutant hepatitis B virus onco-protein HBx without cysteine residues](/preview/png/9279551.png)
چکیده انگلیسی
Chronic infection of the hepatitis B virus (HBV) is one of the causes leading to liver cancer. The 3.2Â kb genome of HBV encodes four proteins: core antigen, surface antigen, a DNA polymerase and the X protein (HBx). The biological functions of HBx are not fully understood. It has been shown that HBx is a potent trans-activator, which activates transcription of many cellular and viral promoters indirectly via protein-protein interactions. These transactivating activities of HBx may contribute to the development of hepatocellular carcinoma. In this paper a truncated mini-HBx(-Cys) (18-142) protein, where the cysteines had been either deleted or substituted by serines, was constructed by site-directed mutagenesis and overexpressed as a 6xHis fusion protein in Escherichia coli. The 6xHis-mini-HBx(-Cys) protein was isolated from inclusion bodies, purified by Ni-affinity chromatography under denaturing conditions and refolded by sequential dialysis. The structure of the 6xHis-mini-HBx(-Cys) protein was analyzed by circular dichroism, fluorescence and one-dimensional NMR spectroscopic assays. The data presented here suggest that HBx is unstructured but has a propensity to gain secondary structure under specific experimental conditions. Its conformational flexibility might partially explain its functional complexity, namely its capacity to interact with a wide array of signaling proteins, transcriptional regulators and nucleic acids.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 126, Issues 1â2, June 2005, Pages 65-74
Journal: Journal of Virological Methods - Volume 126, Issues 1â2, June 2005, Pages 65-74
نویسندگان
Edmilson Rui, Patricia Ribeiro de Moura, Kaliandra de Almeida Gonçalves, Jörg Kobarg,