کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9286677 1594274 2005 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Accumulation of p53 in response to adenovirus early region 1A sensitizes human cells to tumor necrosis factor alpha-induced apoptosis
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Accumulation of p53 in response to adenovirus early region 1A sensitizes human cells to tumor necrosis factor alpha-induced apoptosis
چکیده انگلیسی
Many tumor cells are resistant to tumor necrosis factor alpha (TNFα)-induced apoptosis. Adenovirus early region 1A (AdE1A) sensitizes the otherwise resistant cells to TNFα. AdE1A also stabilizes the p53 protein. The present study demonstrates a correlation between AdE1A-induced sensitization and stabilization of p53 in TNFα-induced apoptosis since the N-terminal and CR2 regions, the binding sites for CBP/p300, Rb and 26S proteasome regulatory components, are required for both these actions of AdE1A. TNFα does not induce apoptosis and AdE1A fails to sensitize TNFα cytotoxicity in p53-negative cells. However, introduction of exogenous p53 overcomes the cellular resistance to TNFα toxicity and enhances AdE1A sensitization, demonstrating that AdE1A sensitizes TNFα-induced apoptosis by its stabilization of p53. A proteasome inhibitor, lactacystin, enhances TNFα cytotoxicity in p53-positive and -negative cells, suggesting that accumulation of cellular proteins other than p53 might also regulate the cellular response to TNFα signaling.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 340, Issue 2, 30 September 2005, Pages 285-295
نویسندگان
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