کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9288374 | 1227471 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The flavivirus-conserved penta-nucleotide in the 3â² stem-loop of the West Nile virus genome requires a specific sequence and structure for RNA synthesis, but not for viral translation
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A reporting replicon of West Nile virus (WN) was used to distinguish between the function of the 3â² untranslated region (UTR) in viral translation and RNA replication. Deletions of various regions of the 3â² UTR of the replicon did not significantly affect viral translation, but abolished RNA replication. A systematic mutagenesis showed that the flavivirus-conserved penta-nucleotide (5â²-CACAG-3â² located at the top of the 3â² stem-loop of the genome) requires a specific sequence and structure for WN RNA synthesis, but not for viral translation. (i) Basepair structure and sequence at the 1st position of the penta-nucleotide are critical for RNA replication. (ii) The conserved nucleotides at the 2nd, 3rd, and 5th positions, but not at the 4th position of the penta-nucleotide, are essential for RNA synthesis. (iii) The nucleotide U (which is partially conserved in the genus Flavivirus) immediately downstream of the penta-nucleotide is not essential for viral replication.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 331, Issue 2, 20 January 2005, Pages 375-386
Journal: Virology - Volume 331, Issue 2, 20 January 2005, Pages 375-386
نویسندگان
Mark Tilgner, Tia S. Deas, Pei-Yong Shi,