کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9289348 | 1594411 | 2005 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inhibition of virion-associated IPNV RNA polymerase, VP1, by radiolabeled nucleotide analogs
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
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چکیده انگلیسی
Infectious pancreatic necrosis virus (IPNV) is a bi-segmented, dsRNA virus of the Birnaviridae family. The structural protein VP1 has been postulated as the RNA-dependent RNA polymerase (RdRp), but its transcriptional activity has not been unequivocally identified from viral particles. Here, we assayed partially purified IPNV in an in vitro RNA synthesis system. To test the RdRp, dialdehyde-nucleotide analogs were used to covalently inhibit the polymerase-associated activity. Our results showed that dialdehyde-nucleotide analogs completely abrogated IPNV in vitro RNA synthesis. The protein involved in this process was identified as viral VP1, since: (a) after incubation of IPNV with [α-32P]2â²,3â²-dialdehyde-UTP, labeled VP1 protein was identified and (b) VP1 was unable to bind [α-32P]GTP when particles were preincubated with 2â²,3â²-dialdehyde-ATP. Thus, within viral particles, inhibition of the transcriptional activity is a result of the binding of 2â²,3â²-dialdehyde-nucleotide analogs to the RdRp, VP1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 112, Issues 1â2, September 2005, Pages 132-135
Journal: Virus Research - Volume 112, Issues 1â2, September 2005, Pages 132-135
نویسندگان
Rodrigo A. Villanueva, Maritza Guacucano, Jacqueline Pizarro, Ana MarÃa Sandino,