کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9296484 | 1233535 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Decreased synthesis of glomerular adrenomedullin in patients with IgA nephropathy
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کلمات کلیدی
PBSCCRSSCRIAmesangial proliferative glomerulonephritiscDNA - cDNAComplementary DNA - DNA تکمیلیIgAN - Iganadrenomedullin - آدرنومدولینstandard saline citrate - استاندارد سدیم سیتراتradioimmunoassay - رادیوایمونواسیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریCreatinine clearance rate - میزان ترخیص کراتینینIgA nephropathy - نفروپاتی ایمونوگلوبین آ، سندرم برگرpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمراز
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
پزشکی و دندانپزشکی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Decreased synthesis of glomerular adrenomedullin in patients with IgA nephropathy Decreased synthesis of glomerular adrenomedullin in patients with IgA nephropathy](/preview/png/9296484.png)
چکیده انگلیسی
Adrenomedullin (AM) immunostaining and gene expression have seldom been measured in human kidneys. Because previous studies have shown that AM exerts antiproliferative effects on rat mesangial cells in vitro and that urine AM levels are decreased in patients with chronic glomerulonephritis, we measured glomerular AM and its gene expression in patients with primary IgA nephropathy (IgAN). Glomerular AM was measured by immunohistochemical staining, and glomerular AM mRNA was measured by in situ hybridization. Plasma and urine AM were measured by radioimmunoassay. The results showed that both the intensity of immunostaining for glomerular AM and the glomerular expression of AM mRNA were significantly decreased in IgAN patients compared with normal controls (both P < .05). Similar results were not observed in patients with non-IgA MsPGN. Glomerular AM immunostaining and glomerular AM mRNA expression were significantly correlated (P < .001), and both were negatively correlated with the number of glomerular cells (P < .05 and < .01, respectively). Both glomerular AM immunostaining and glomerular AM mRNA expression were correlated with urine AM levels (both P < .001), but not with plasma AM levels. The urine AM level was significantly lower in IgAN patients than in normal controls (P < .01), whereas the plasma level was not different between the 2 groups. Our findings indicate that glomerular production of AM was decreased in patients with IgA nephropathy and that this lack of glomerular AM may be related to the pathogenesis of this mesangial disease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Laboratory and Clinical Medicine - Volume 145, Issue 5, May 2005, Pages 233-238
Journal: Journal of Laboratory and Clinical Medicine - Volume 145, Issue 5, May 2005, Pages 233-238
نویسندگان
Mei-Chuan Kuo, Hung-Tien Kuo, Yi-Wen Chiu, Jer-Ming Chang, Jinn-Yuh Guh, Yung-Hsiung Lai, Hung-Chun Chen,