کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9425648 | 1295885 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Long-term effects of diazepam and phenobarbital treatment during development on GABA receptors, transporters and glutamic acid decarboxylase
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کلمات کلیدی
SDSglutamic acid decarboxylase-67BZsGATSaRNAN-[2-hydroxyethyl] piperazine-N′-[2-ethanesulfonic acid]GABA transportersPKCAEDsGad67GADHEPESglutamic acid decarboxylase - glutamic acid dearboxylaseAntisense RNA - RNA آنتیسنسγ-aminobutyric acid - اسید γ-آمینوبوتیریکBenzodiazepines - بنزودیازپینهاRNA amplification - تقویت RNAantiepileptic drugs - داروهای ضدصرع dentate - دندانه دارdiazepam - دیازپامpostnatal day - روز پس از زایمانsodium dodecyl sulfate - سدیم دودسیل سولفاتPhenobarbital - فنوباربیتالPipes - لوله هایChronic - مزمنRat - موش صحراییHippocampus - هیپوکامپ Vehicle - وسیله نقلیهProtein kinase C - پروتئین کیناز سیPostnatal - پس از زایمانGABA - گاباGABAR - گابارGABAA receptors - گیرنده های GABAA
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Long-term effects of diazepam and phenobarbital treatment during development on GABA receptors, transporters and glutamic acid decarboxylase Long-term effects of diazepam and phenobarbital treatment during development on GABA receptors, transporters and glutamic acid decarboxylase](/preview/png/9425648.png)
چکیده انگلیسی
Diazepam (DZ) and phenobarbital (PH) are commonly used to treat early-life seizures and act on GABAA receptors (GABAR). The developing GABAergic system is highly plastic, and the long-term effects of postnatal treatment with these drugs on the GABAergic system has not been extensively examined. In the present study, we investigated the effects of prolonged DZ and PH treatment during postnatal development and then discontinuation on expression of a variety of genes involved in GABAergic neurotransmission during adulthood. Rat pups were treated with DZ, PH or vehicle from postnatal day (P) 10-P40 and then the dose was tapered for 2 weeks and terminated at P55. Expression of GABAR subunits, GABAB receptor subunits, GABA transporters (GAT) and GABA synthesizing enzymes (glutamic acid decarboxylase: GAD) mRNAs in hippocampal dentate granule neurons (DGNs) were analyzed using antisense RNA amplification at P90. Protein levels for the α1 subunit of GABAR, GAD67, GAT1 and 3 were also assessed using Western blotting. At P90, mRNA expression for GAT-1, 3, 4, GABAR subunits α4, α6, β3, δ and θ and GABAB receptor subunit R1 was increased and mRNA expression for GAD65, GAD67 and GABAR subunits α1 and α3 were decreased in DGNs of rats treated with DZ and PH. The current data suggest that prolonged DZ and PH treatment during postnatal development causes permanent alterations in the expression of hippocampal GABA receptor subunits, GATs and GAD long after therapy has ended.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 132, Issue 2, 2005, Pages 399-407
Journal: Neuroscience - Volume 132, Issue 2, 2005, Pages 399-407
نویسندگان
Y.H. Raol, G. Zhang, E.C. Budreck, A.R. Brooks-Kayal,