کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9426578 | 1295927 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Unusual astrocyte reactivity caused by the food mycotoxin ochratoxin A in aggregating rat brain cell cultures
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کلمات کلیدی
OTA15-deoxy-Δ12,14 prostaglandin J2Br-cAMP15d-PGJ2Heme oxygenase-1ISHPPARγiNOSRT-PCRGFAPHO-1PBSIn situ hybridization - Hybridization در محلBrain inflammation - التهاب مغزOchratoxin A - اکراتوکسین Aanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceReverse transcriptase - ترانس کریپتاز معکوس یا وارونویسDIV - دیوday in vitro - روز in vitroinducible nitric oxide synthase - سنتاز اکسید نیتریک القاییlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH Phosphate-buffered saline - محلول نمک فسفات با خاصیت بافریNitric oxide - نیتریک اکسیدReverse transcriptase-polymerase chain reaction - واکنش زنجیره ای واکنش زنجیره ای واکنش زنجیره ایGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالGlutamine synthase - گلوتامین سنتازGliosis - گلیوزperoxisome proliferator-activated receptor γ - گیرنده پروتئین کننده پروکسیوم فعال γ
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Ochratoxin A (OTA), a mycotoxin and widespread food contaminant, is known for its patent nephrotoxicity and potential neurotoxicity. Previous observations in vitro showed that in the CNS, glial cells were particularly sensitive to OTA. In the search for the molecular mechanisms underlying OTA neurotoxicity, we investigated the relationship between OTA toxicity and glial reactivity, in serum-free aggregating brain cell cultures. Using quantitative reverse transcriptase-polymerase chain reaction to analyze changes in gene expression, we found that in astrocytes, non cytotoxic concentrations of OTA down-regulated glial fibrillary acidic protein, while it up-regulated vimentin and the peroxisome proliferator-activated receptor-γ expression. OTA also up-regulated the inducible nitric oxide synthase and the heme oxygenase-1. These OTA-induced alterations in gene expression were more pronounced in cultures at an advanced stage of maturation. The natural peroxisome proliferator-activated receptor-γ ligand, 15-deoxy-Î12,14 prostaglandin J2, and the cyclic AMP analog, bromo cyclic AMP, significantly attenuated the strong induction of peroxisome proliferator-activated receptor-γ and inducible nitric oxide synthase, while they partially reversed the inhibitory effect of OTA on glial fibrillary acidic protein. The present results show that OTA affects the cytoskeletal integrity of astrocytes as well as the expression of genes pertaining to the brain inflammatory response system, and suggest that a relationship exists between the inflammatory events and the cytoskeletal changes induced by OTA. Furthermore, these results suggest that, by inducing an atypical glial reactivity, OTA may severely affect the neuroprotective capacity of glial cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 134, Issue 3, 2005, Pages 771-782
Journal: Neuroscience - Volume 134, Issue 3, 2005, Pages 771-782
نویسندگان
M.-G. Zurich, S. Lengacher, O. Braissant, F. Monnet-Tschudi, L. Pellerin, P. Honegger,