کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9487526 | 1330759 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation and modification of existing methods for the quantification of exopolysaccharides in milk-based media
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Two EPS assay procedures were evaluated using a ropy culture (Lactobacillus delbrueckii subsp. bulgaricus NCFB 2483) grown in a milk-based medium. Reproducible EPS measurements were unachievable because the proteins and lactose in the growth medium interfered with the separation of EPS and phenol-sulphuric acid method. Accurate determination of EPS in a complex system requires an effective regime to separate EPS from non-EPS components in fermented broths. In the development of a new EPS assay procedure, the reliability of the assay was assessed by adding a known amount of dextran to a fresh growth medium. Each processing step was then evaluated based on dextran recovery. Key improvements made to the EPS assay procedures included the use of Flavourzyme for protein hydrolysis; optimizing ethanol concentration to prevent lactose crystallization yet allowing complete EPS precipitation; and a suitable centrifugation regime to minimize EPS loss. The improved EPS assay gave reproducible results (5% coefficient of variation) with greater accuracy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Research International - Volume 38, Issue 6, July 2005, Pages 605-613
Journal: Food Research International - Volume 38, Issue 6, July 2005, Pages 605-613
نویسندگان
Kelvin K.T. Goh, Derek R. Haisman, Richard H. Archer, Harjinder Singh,