کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9573243 | 1388893 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of phosphatidylglycerol on molecular organization of photosystem I
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Phosphatidylglycerol (PG) is the only anionic phospholipid in photosynthetic membrane. In this study, photosystem I (PSI) particles obtained from plant spinach were reconstituted into PG liposomes at a relatively high concentration. The results from visible absorption, fluorescence emission, and circular dichroism (CD) spectra reveal an existence of the interactions of PSI with PG. PG effect causes blue-shift and intensity decrease of Chl a peak bands in the absorption and 77 K fluorescence emission. The visible CD spectra indicate that the excitonic interactions for Chl a and Chl b molecules were enhanced upon reconstitution. Furthermore, more or less blue- or red-shift of the peaks characterized by Chl a, Chl b, and carotenoid molecules are also occurred. Simultaneously, an increase in α-helix and a decrease particularly in the disordered conformations of protein secondary structures are observed. In addition, the same effect also leads to somewhat more tryptophan (Trp) residues exposed to the polar environment. These results demonstrate that some alteration of molecular organization occurs within both the external antenna LHCI and PSI core complex after PSI reconstitution.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 115, Issue 1, 1 May 2005, Pages 19-27
Journal: Biophysical Chemistry - Volume 115, Issue 1, 1 May 2005, Pages 19-27
نویسندگان
Zhenle Yang, Xinhua Su, Feng Wu, Yandao Gong, Tingyun Kuang,