کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9600480 | 1404691 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Non-destructive Analysis of the Nuclei of Transgenic Living Cells Using Laser Tweezers and Near-infrared Raman Spectroscopic Technique
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Non-destructive Analysis of the Nuclei of Transgenic Living Cells Using Laser Tweezers and Near-infrared Raman Spectroscopic Technique Non-destructive Analysis of the Nuclei of Transgenic Living Cells Using Laser Tweezers and Near-infrared Raman Spectroscopic Technique](/preview/png/9600480.png)
چکیده انگلیسی
Transgenic cell lines of loblolly pine (Pinus taeda L.) were analyzed by a compact laser-tweezers-Raman-spectroscopy (LTRS) system in this investigation. A low power diode laser at 785Â nm was used for both laser optical trapping of single transgenic cells and excitation for near-infrared Raman spectroscopy of the nuclei of synchronized cells, which were treated as single organic particles, at the S-phase of the cell cycle. Transgenic living cells with gfp and uidA genes were used as biological samples to test this LTRS technique. As expected, different Raman spectra were observed from the tested biological samples. This technique provides a high sensitivity and enables real-time spectroscopic measurements of transgenic cell lines. It could be a valuable tool for the study of the fundamental cell and molecular biological process by trapping single nucleus and by providing a wealth of molecular information about the nuclei of cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Genomics, Proteomics & Bioinformatics - Volume 3, Issue 3, 2005, Pages 169-178
Journal: Genomics, Proteomics & Bioinformatics - Volume 3, Issue 3, 2005, Pages 169-178
نویسندگان
Wei Tang, Ronald J. Newton, Chang-An Xie, Yong-Qing Li, Nicki Whitley,