کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9604240 | 43608 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Novel detection system for biomolecules using nano-sized bacterial magnetic particles and magnetic force microscopy
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Novel detection system for biomolecules using nano-sized bacterial magnetic particles and magnetic force microscopy Novel detection system for biomolecules using nano-sized bacterial magnetic particles and magnetic force microscopy](/preview/png/9604240.png)
چکیده انگلیسی
A system for streptavidin detection using biotin conjugated to nano-sized bacterial magnetic particles (BMPs) has been developed. BMPs, isolated from magnetic bacteria, were used as magnetic markers for magnetic force microscopy (MFM) imaging. The magnetic signal was obtained from a single particle using MFM without application of an external magnetic field. The number of biotin conjugated BMPs (biotin-BMPs) bound to streptavidin immobilized on the glass slides increased with streptavidin concentrations up to 100Â pg/ml. The minimum streptavidin detection limit using this technique is 1Â pg/ml, which is 100 times more sensitive than a conventional fluorescent detection system. This is the first report using single domain nano-sized magnetic particles as magnetic markers for biosensing. This assay system can be used for immunoassay and DNA detection with high sensitivities.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 120, Issue 3, 21 November 2005, Pages 308-314
Journal: Journal of Biotechnology - Volume 120, Issue 3, 21 November 2005, Pages 308-314
نویسندگان
Yosuke Amemiya, Tsuyoshi Tanaka, Brandon Yoza, Tadashi Matsunaga,