کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9604303 43617 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High through-put characterization of insect morphocryptic entities by a non-invasive method using direct-PCR of fecal DNA
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
High through-put characterization of insect morphocryptic entities by a non-invasive method using direct-PCR of fecal DNA
چکیده انگلیسی
The development of non-invasive molecular techniques is currently increasing, particularly in the fields of behavioural ecology and conservation genetics of mammals. Surprisingly, genetic studies of Arthropods and particularly the insects have not benefited yet from the contributions that non-invasive methods have made. Here, we outline a strategy for identifying phytophagous insect genetic entities based on direct-PCR of fecal DNA combined with double strand conformation polymorphism (DSCP) typing. This allows the differentiation of morphocryptic entities within the species Ceutorhynchus assimilis (Coleoptera: Curculionidae), a candidate biocontrol agent of a noxious weed. The results obtained clearly demonstrate the potential for this method to provide a valuable means for genetic and ecological studies of Arthropods.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 119, Issue 1, 22 September 2005, Pages 15-19
نویسندگان
, , ,