کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9604331 | 43595 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High level expression of functionally active human lactoferrin in silkworm larvae
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: High level expression of functionally active human lactoferrin in silkworm larvae High level expression of functionally active human lactoferrin in silkworm larvae](/preview/png/9604331.png)
چکیده انگلیسی
In this paper, recombinant human lactoferrin (rhLf) was expressed very well using Bombyx mori nuclear polyhedrosis baculovirus expression system. Infection of silkworm larvae with recombinant virus, vBm-hLf, the rhLf was efficiently secreted into larvae hemolymph and the concentration of product purified was about 65 μg/ml. The isolated rhLf molecular mass was â¼78 kDa, lower than that of the human lactoferrin (hLf) standards, which may be due to incomplete glycosylation or protein degradation. Furthermore, the rhLf was characterized and its biological activities were evaluated by in vivo bioassay using dextran sodium sulfate (DSS)-induced colitis mouse model that mimics some characteristics of colitis disease in human. We conclude that silkworm expression system can be used successfully to express functional human lactoferrin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 118, Issue 3, 22 August 2005, Pages 246-256
Journal: Journal of Biotechnology - Volume 118, Issue 3, 22 August 2005, Pages 246-256
نویسندگان
Tao Liu, Yao-Zhou Zhang, Xiang-Fu Wu,