کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9604383 43628 2005 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression and characterisation in E. coli of mutant forms of saporin
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Expression and characterisation in E. coli of mutant forms of saporin
چکیده انگلیسی
In the present communication, we report on the expression and characterisation in Escherichia coli of mutant derivatives of saporin, a type 1 ribosome-inactivating protein from Saponaria officinalis L. The effects of substitution of Glu 176 with Lys and those of deletion of 19 amino acids at the C-terminal were evaluated both in vivo, testing the influence of expressed proteins on bacterial growth and in vitro measuring their N-glycosidase and supercoiled DNA relaxation activities. Results indicate that both modifications of the wild-type protein abolish its toxicity to bacterial cells and impair its enzymatic activity on polynucleotide substrates, either RNA or DNA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 117, Issue 3, 25 May 2005, Pages 263-266
نویسندگان
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