کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9604391 | 43616 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Influence of DNA stiffness in protein-DNA recognition
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Influence of DNA stiffness in protein-DNA recognition Influence of DNA stiffness in protein-DNA recognition](/preview/png/9604391.png)
چکیده انگلیسی
Protein-DNA recognition plays an essential role in the regulation of gene expression. The protein-DNA binding specificity is based on direct atomic contacts between protein and DNA and/or the conformational properties of DNA. In this work, we have analyzed the influence of DNA stiffness (E) to the specificity of protein-DNA complexes. The average DNA stiffness parameters for several protein-DNA complexes have been computed using the structure based sequence dependent stiffness scale. The relationship between DNA stiffness and experimental protein-DNA binding specificity has been brought out. We have investigated the importance of DNA stiffness with the aid of experimental free energy changes (ÎÎG) due to binding in several protein-DNA complexes, such as, ETS proteins, 434, λ, Mnt and trp repressors, 434 cro protein, EcoRV endonuclease V and zinc fingers. We found a correlation in the range 0.65-0.97 between ÎÎG and E in these examples. Further, we have qualitatively analyzed the effect of mutations in the target sequence of λ repressor and we observed that the DNA stiffness could correctly identify 70% of the correct bases among the considered nine positions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 117, Issue 2, 4 May 2005, Pages 137-145
Journal: Journal of Biotechnology - Volume 117, Issue 2, 4 May 2005, Pages 137-145
نویسندگان
M. Michael Gromiha,