کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9604396 | 43616 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Novel interface-binding chloroperoxidase for interfacial epoxidation of styrene
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Novel interface-binding chloroperoxidase for interfacial epoxidation of styrene Novel interface-binding chloroperoxidase for interfacial epoxidation of styrene](/preview/png/9604396.png)
چکیده انگلیسی
A unique interface-binding chloroperoxidase (CPO) was developed and examined for interfacial biocatalysis. Native CPO was conjugated with polystyrene (PS) to form a surfactant-like structure that self assembled at oil-water interfaces. While enantioselectivity of the enzyme was maintained, the interfacial assembly of the enzyme improved its overall catalytic efficiency as compared to that observed with the enzyme contained in the bulk aqueous phase. The PS conjugated CPO (PS-CPO) showed a 2.5-fold enhancement of enzyme productivity versus native CPO under batch reaction conditions for the epoxidation of styrene, whereas a 25-fold improvement was realized in a continuous feeding reaction to reach a productivity of 10 μmol hâ1 mg proteinâ1. The interface-binding enzyme also demonstrated several other advantages such as suppressing unwanted side reactions including the hydrolysis of styrene epoxide products, stabilizing the enzyme by limiting its exposure to both the oxidant H2O2 and epoxide products, and alleviating the deactivating effect of interfacial stress on enzymes by functioning as a surfactant.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 117, Issue 2, 4 May 2005, Pages 195-202
Journal: Journal of Biotechnology - Volume 117, Issue 2, 4 May 2005, Pages 195-202
نویسندگان
Guangyu Zhu, Ping Wang,