کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9604450 | 43641 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Improved stabilization of chemically aminated enzymes via multipoint covalent attachment on glyoxyl supports
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Improved stabilization of chemically aminated enzymes via multipoint covalent attachment on glyoxyl supports Improved stabilization of chemically aminated enzymes via multipoint covalent attachment on glyoxyl supports](/preview/png/9604450.png)
چکیده انگلیسی
In both cases, the immobilization of the chemically modified enzymes improved the enzyme stability compared to the stability of the immobilized but non-modified enzyme (by a four-fold factor in the case of PGA and a 20-fold factor in the case of GA). The chemical modification presented a deleterious effect on soluble enzyme stability. Therefore, the improved stability should be related to a higher multipoint covalent attachment, involving both the lysine amino groups and also the new amino groups chemically introduced on the enzyme. Moreover, the lower pKa of the new amino groups permitted to immobilize the enzyme under milder conditions. In fact, the aminated proteins could be immobilized even at pH 9, while the non-modified enzymes could only be immobilized at pH over 10.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 116, Issue 1, 2 March 2005, Pages 1-10
Journal: Journal of Biotechnology - Volume 116, Issue 1, 2 March 2005, Pages 1-10
نویسندگان
Fernando López-Gallego, Tamara Montes, Manuel Fuentes, Noelia Alonso, Valeria Grazu, Lorena Betancor, Jose M. Guisán, Roberto Fernández-Lafuente,