کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9605763 | 44156 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Spectroscopic studies on the interaction of Safranine T with DNA in β-cyclodextrin and carboxymethyl-β-cyclodextrin
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Spectroscopic studies on the interaction of Safranine T with DNA in β-cyclodextrin and carboxymethyl-β-cyclodextrin Spectroscopic studies on the interaction of Safranine T with DNA in β-cyclodextrin and carboxymethyl-β-cyclodextrin](/preview/png/9605763.png)
چکیده انگلیسی
The interaction of Safranine T (ST) with cyclodextrins (CDs) including β-cyclodextrin (β-CD) and carboxymethyl-β-cyclodextrin (CM-β-CD) and DNA in aqueous solution is studied by UV-vis absorption and steady-state fluorescence technique. The interactive model of ST with double stranded DNA has been investigated by means of the inclusive action of β-CD and CM-β-CD. Based on the changes of absorption, fluorescence and resonance light scattering (RLS) spectra, the intrinsic binding constant (Kap) and the binding site number (n) of ST with DNA and the inclusion complexes ST-CD with DNA are obtained in the case of 20 mmol/L Tris-HCl buffer solution (pH 7.2). According to the experimental results, it can be inferred that the interactive model of dimer ST with DNA is “electrostatic binding”, while the monomer ST with DNA is “intercalative binding”.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 169, Issue 2, 15 January 2005, Pages 153-158
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 169, Issue 2, 15 January 2005, Pages 153-158
نویسندگان
Guomei Zhang, Yuehong Pang, Shaomin Shuang, Chuan Dong, Martin M.F. Choi, Diansheng Liu,