کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9616736 | 48866 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Synthesis of super-high-molecular-weight poly-γ-glutamic acid by Bacillus subtilis subsp. chungkookjang
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
کاتالیزور
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Synthesis of super-high-molecular-weight poly-γ-glutamic acid by Bacillus subtilis subsp. chungkookjang Synthesis of super-high-molecular-weight poly-γ-glutamic acid by Bacillus subtilis subsp. chungkookjang](/preview/png/9616736.png)
چکیده انگلیسی
Poly-γ-glutamic acid (PGA) with high molecular weight is a most promising biomaterial in industrial uses; however, it generally diverse in molecular structure and co-produced with polysaccharides and various other biopolymers. In this study, it was ascertained that Bacillus subtilis subsp. chungkookjang cells are superior to B. subtilis (natto) cells as the biocatalyst for the synthesis of super-high-molecular-weight PGA (over 2000 k). We effectively purified PGA and fractionated according to its molecular weight by anion-exchange chromatography, and further developed a simple method for determination of the molecular weight of PGA on the basis of numbers of glutamate monomers generated by hydrolysis and a free amino group quantified with 1-fluoro-2,4-dinitrobenzene (FDNB). The molecular weight determination with FDNB was available even for a super-high-molecular-weight PGA, e.g. the 2000-k polymer. Super-high-molecular-weight PGAs (average 2000 k and 7000 k), which were synthesized by the use of B. subtilis subsp. chungkookjang cells in the presence of a high concentration of ammonium sulfate, were rich in l-glutamate rather than in the d-enantiomer.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 35, Issues 4â6, 1 September 2005, Pages 128-133
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 35, Issues 4â6, 1 September 2005, Pages 128-133
نویسندگان
Chung Park, Jae-Chul Choi, Yoon-Ho Choi, Hisaaki Nakamura, Kazuya Shimanouchi, Terumi Horiuchi, Haruo Misono, Tomomitsu Sewaki, Kenji Soda, Makoto Ashiuchi, Moon-Hee Sung,