کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9634230 | 463409 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Design variations of a polymer-enzyme composite biosensor for glucose: Enhanced analyte sensitivity without increased oxygen dependence
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
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چکیده انگلیسی
The glucose sensitivity and oxygen dependence of a variety of implantable biosensors based on glucose oxidase (GOx), incorporating an electrosynthesized poly-o-phenylenediamine (PPD) permselective barrier on 125-μm diameter Pt disks (PtD) and cylinders (PtC, 1-mm length), were measured and compared. Full glucose calibrations and experimental monitoring of solution oxygen concentration allowed us to determine apparent Michaelis-Menten parameters for glucose and oxygen. In the linear region of glucose response, the most sensitive biosensor design studied was PtD/PPD/GOx (enzyme deposited over polymer) that was â¼20 times more sensitive than the more widely used PtC/GOx/PPD (enzyme immobilized before polymer deposition) configuration. The oxygen dependence, quantified as KM(O2), of both active and less active designs was surprisingly similar, a finding that could be rationalized in terms of an increase in KM(G) with increased enzyme loading. The PtD/PPD/GOx design will now enable us to explore glucose concentration dynamics in smaller and layered brain regions with good sensitivity and minimal interference from fluctuations in tissue pO2.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Electroanalytical Chemistry - Volume 580, Issue 2, 1 July 2005, Pages 193-202
Journal: Journal of Electroanalytical Chemistry - Volume 580, Issue 2, 1 July 2005, Pages 193-202
نویسندگان
Colm P. McMahon, Sarah J. Killoran, Robert D. O'Neill,