Surface plasmon resonance immunosensor for the detection of Yersinia enterocolitica

An immunosensor based on surface plasmon resonance (SPR) using protein G was developed for the detection of Yersinia enterocolitica. A protein G layer was fabricated by binding chemically to self-assembly monolayer (SAM) of 11-mercaptoundecanoic acid (MUA) on gold (Au) surface. The formation of protein G layer on Au surface modified with 11-MUA and the binding of antibody and antigen in series were confirmed by SPR spectroscopy. Consequently, an immunosensor based on SPR for the detection of Y. enterocolitica using protein G was developed with a detection range of 102 to 107 CFU/mL. The proposed fabrication technique of a SPR immunosensor using protein G on SAM could be applied to construct the other kind of immunosensors and protein chips.