کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9743406 | 1491189 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of a rapid liquid chromatography tandem mass spectrometry method for the determination of lisinopril, applicable for a bioequivalence study, employing a 96-well format solid phase extraction protocol
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A rapid liquid chromatography/tandem mass spectrometry (LC/MS-MS) method was developed for the determination of lisinopril in human plasma. Lisinopril and the internal standard enalaprilat (IS) were extracted from human plasma by semi-automated solid phase extraction (SPE) using a 96-well format extraction plate. Initially, a 1:1 plasma: acetonitrile (ACN) mixture was prepared and vortexed to achieve protein precipitation. After centrifugation, an aliquot of the supernatant water/ACN solvent mixture was evaporated. The residue was dissolved in a certain volume of a reconstitution solution, which was passed through the extraction plate and the eluent was analyzed by combined reversed phase liquid chromatography tandem mass spectrometry with positive ion electrospray ionization using multiple reactions monitoring (MRM). The method was proved to be sensitive and specific for both drugs and its statistical evaluation revealed excellent linearity for the range of concentrations 2.0-200.0 ng mLâ1 and very good accuracy, and inter- and intraday precisions. The proposed method enables the rapid and reliable determination of lisinopril in pharmacokinetic or bioequivalence studies after per os administration of 20 mg tablet formulations of lisinopril and it was applied in such study from our laboratory.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 551, Issues 1â2, 17 October 2005, Pages 177-183
Journal: Analytica Chimica Acta - Volume 551, Issues 1â2, 17 October 2005, Pages 177-183
نویسندگان
Constantinos Kousoulos, Georgia Tsatsou, Yannis Dotsikas, Yannis L. Loukas,