Determination of cercosporin (CER) phytotoxin isolated from infected peanut leaves by using adsorptive stripping square wave voltammetry

The recovery percentage of CER using ASSWV was determined by employing healthy peanut leaf tissue spiked with standard CER. The application of standard addition method was used to determine the recovery percentage as well as the CER contamination level in infected extracts. A recovery percentage of 93.27% was obtained with a relative standard deviation of 21.8% for a solution concentration of 1.04 × 10−6 mol dm−3 (n = 11). The limit of detection for a signal to noise ratio of 3:1 was about 6 ppb and the quantitation limit was about 50 ppb. The pondered relative standard deviation of 9.5% was calculated for the ASSWV method. Results found for CER contamination level in extracts through the electroanalytical method here proposed are in reasonable agreement with those values determined by using HPLC measurements.