کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9743625 | 1491195 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparison and development of two different solid phase chemiluminescence ELISA for the determination of albumin in urine
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Comparison and development of two different solid phase chemiluminescence ELISA for the determination of albumin in urine Comparison and development of two different solid phase chemiluminescence ELISA for the determination of albumin in urine](/preview/png/9743625.png)
چکیده انگلیسی
Two chemiluminescence enzyme-linked immunosorbent assay (ELISA) methods based on avidin-biotin system and Fluorescein-iso-thiocyanate (FITC)-anti-FITC system as two different solid phase for the determination of albumin in urine were optimized, characterized and compared. Avidin or anti-FITC antibody was coated in the microplates to provide a universal solid phase which improved the variability and sensitivity. Alkaline phosphatase (ALP) labeled albumin and albumin from standard or patient samples compete for biotinylated-antibody or FITC labeled antibody binding sites. Enzyme activity in the bound fraction was detected with the chemiluminescence substrate 4-methoxy-4-(3-phosphatephenyl)-spiro-(1,2-dioxetane-3,2â²-adamantane) (AMPPD). The influence of several physico-chemical parameters, such as incubation time, detergent concentration and solid phase conditions were also studied. For the two solid phase, both the linear range and the limit of detection of albumin were 0.15-15 and 0.089 μg/mL, compared with the commercially ELISA kit, a good correlation were obtained. Moreover, three different solid phases include avidin-biotin system, FITC-anti-FITC system and the anti-albumin Ab directly coated the microtitre plates were compared mainly from the cost and precision, the results showed: (1) when the avidin-biotin system as solid phase, the amounts of the antibody used was only the 1/10 than those of the anti-FITC antibody as solid phase and antibody directly coated the microtiter plates; (2) the C.V. of these two solid-phase CL ELISA methods were lower than that of antibody directly coated solid phase.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 541, Issues 1â2, 13 June 2005, Pages 197-205
Journal: Analytica Chimica Acta - Volume 541, Issues 1â2, 13 June 2005, Pages 197-205
نویسندگان
Lixia Zhao, Jin-Ming Lin, Zhenjia Li,