کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9743695 | 1491197 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A crosslinked matrix of thylakoids coupled to the fluorescence transducer in order to detect herbicides
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A new method to detect photosynthetic herbicides has been developed. It is based on the fluorescence activity of spinach thylakoids immobilised in a matrix of crosslinked albumin-glutaraldehyde. Among all the tested parameters, it was the area over the fluorescence curve (FA) which showed the greatest modification in the presence of herbicides. So that was therefore selected for the experiments. The optimal area was measured at 25 °C in a 10 mM phosphate buffer, pH 5.5. After immobilisation, the value of fluorescence area retained 77% of the activity after a 240-day storage at â18 °C. A good operational stability was given by the measuring of the fluorescence area every 10 min during 180 min at +5 and +25 °C. The limits of detection were determined for various photosynthesis inhibiting herbicides (corresponding to a 10% inhibition of FA). The greatest sensitivity for the detection of the phenylurea diuron (0.35 μg/L) was recorded and the order of sensitivity among the triazines and triazinones chemical classes were, respectively, metribuzin (2 μg/L), atrazine (3.5 μg/L), and cyanazine (10 μg/L). It was also possible to detect low concentrations of diuron in environmental water samples except for highly concentrated salt waters which modified the fluorescence activity of the thylakoid matrix. The results suggest that this system could be useful for monitoring of pollutants and environmental pre-screening.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 539, Issues 1â2, 10 May 2005, Pages 263-269
Journal: Analytica Chimica Acta - Volume 539, Issues 1â2, 10 May 2005, Pages 263-269
نویسندگان
Pierre Euzet, Maria Teresa Giardi, Regis Rouillon,