کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9743803 | 1491199 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Flow analysis of p-aminophenyl phosphate with a gold nanoelectrode ensemble based detector
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
An alkaline phosphatase (AP) immobilized gold nanoelectrode ensemble (GNEE) was fabricated by electroless deposition of Au3+ onto the nanopores (d = 50 nm) of polycarbonate membranes and modifying the surface of GNEE successively with a self-assembled monolayer (SAM) of 2-aminoethanethiol, an alkaline phosphatase (AP) layer and a porous film of poly(4-vinylpyridine-co-styrene) (PVP). Performance of the AP-GNEE was studied by monitoring the current responses from the injection of p-aminophenyl phosphate (p-APP) into a flow injection analysis (FIA) system. Optimal conditions such as concentration of AP for immobilization, pH of carrier solution, potential applied for the analysis of p-APP were studied. Calibration graph over a broad dynamic concentration range (0.1 μM to 1.0 mM) gave satisfactory linearity (R2 = 0.986) and a very low detection limit (0.1 μM) confirming the applicability and superiority of the AP-GNEE for p-APP analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 537, Issues 1â2, 29 April 2005, Pages 315-319
Journal: Analytica Chimica Acta - Volume 537, Issues 1â2, 29 April 2005, Pages 315-319
نویسندگان
Tsung-Huang Hsia, Kuo-Tang Liao, Hsuan-Jung Huang,