Supercritical fluid chromatographic analysis for on-line monitoring of hexane removal from soybean oil miscella using liquid carbon dioxide

Liquid carbon dioxide (L-CO2) can be used to separate hexane from hexane/soybean oil (SBO) mixtures (i.e., miscella). An on-line supercritical fluid chromatographic (SFC) method was developed to monitor this separation. L-CO2 (25 °C and 9.31 MPa) was passed through 50 mL of a 25% (w/w) hexane miscella and then directed on-line through a SFC injector. After passing 300-L expanded CO2, the hexane concentrations in the L-CO2 were 0.05% and 0.04% for n-hexane and isohexane, respectively and the residual hexane concentrations in the SBO were 3.8 and 3.3 ppm, respectively. This technique provided real time on-line monitoring of the hexane separation process.