کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9748700 | 1493789 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Capillary high-performance liquid chromatography/electrospray ion trap time-of-flight mass spectrometry using a novel nanoflow gradient generator
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A type of high-performance liquid chromatography (HPLC) based on a novel nanoflow gradient generator (Asymptotic-Trace-10-Port-Valve (AT10PV) nanoGR generator) was developed and coupled with an electrospray ion trap time-of-flight mass spectrometer (ESI-IT-TOF MS). Stability of the nanoflow GR HPLC system was tested at flow rates of 20 and 50 nL/min by using a nanoflow meter. Average flow rates in a 2-h run were 51.2 nL/min with RSD 0.7% and 21.0 nL/min with RSD 1.8%. Repeatability of analysis of the nanoHPLC/ESI-IT-TOF MS system was also tested by injecting 1.0 μL of trypsin digested bovine serum albumin (BSA) (100 fmol) into a monolithic silica-ODS column (30 μm i.d., 150 mm in length) through a packed silica-ODS trapping column (particle size 5 μm, 150 μm i.d., 10 mm in length). At a flow rate of 50 nL/min, the result demonstrated a reasonably good repeatability of peak retention times (RSD: 0.32-1.1%) and base-ion peak areas (RSD: 4.4-6.6%).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1090, Issues 1â2, 7 October 2005, Pages 178-183
Journal: Journal of Chromatography A - Volume 1090, Issues 1â2, 7 October 2005, Pages 178-183
نویسندگان
Shinya Ito, Shinji Yoshioka, Izumi Ogata, Eri Yamashita, Shinji Nagai, Toyoharu Okumoto, Kimihiko Ishii, Masahito Ito, Hironori Kaji, Kunihiko Takao, Kisaburo Deguchi,