کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9748731 | 1493790 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of malachite green and leucomalachite green in carp muscle by liquid chromatography with visible and fluorescence detection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Determination of malachite green and leucomalachite green in carp muscle by liquid chromatography with visible and fluorescence detection Determination of malachite green and leucomalachite green in carp muscle by liquid chromatography with visible and fluorescence detection](/preview/png/9748731.png)
چکیده انگلیسی
A liquid chromatography-VIS/FLD method for the analysis of malachite green (MG) and its major metabolite, leucomalachite green (LMG) in carp muscle has been described. The method consists in an extraction with acetonitrile-buffer mixture followed by partioning with dichloromethane. Clean up and isolation were performed on SCX solid phase extraction (SPE) column. Chromatographic separation was achieved by using phenyl-hexyl column with an isocratic mobile phase consisting of acetonitrile and acetate buffer (0.05 M, pH 4.5) (60:40, v/v). Liquid chromatography with absorbance detector (λ = 620 nm) was used for the determination of MG while LMG was detected by fluorescence detector (λex = 265 nm and λem = 360 nm). The both detectors were connected on-line which allowed direct analysis of a sample extract for MG and LMG without the need for any post-column procedure. The whole method has been validated, according to the EU requirements (Commission Decision 2002/657/EC). Specificity, stability, decision limit (CCα), detection capability (CCβ), accuracy and precision were determined. Average recoveries of MG and LMG from muscle fortified at three levels (0.5, 1 and 2 μg/kg) were 62% (range from 60.4 to 63.5%) and 90% (range from 89.0 to 91.5%), respectively. Relative standard deviations (RSD) of recoveries at all fortification levels were less than 10.9 and 8.6% for MG and LMG, respectively. The calculated CCα for MG and LMG were 0.15 and 0.13 μg/kg, and CCβ were 0.37 and 0.32 μg/kg, complying with the minimum required performance limit (MRPL) of 2 μg/kg (sum of MG and LMG).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1089, Issues 1â2, 30 September 2005, Pages 187-192
Journal: Journal of Chromatography A - Volume 1089, Issues 1â2, 30 September 2005, Pages 187-192
نویسندگان
Kamila Mitrowska, Andrzej Posyniak, Jan Zmudzki,