کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9749154 1493802 2005 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Monitoring bronchodilators with direct injection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Monitoring bronchodilators with direct injection
چکیده انگلیسی
A procedure was developed for the determination of caffeine and theophylline using a C18 column (5 μm, 250 mm × 4.6 mm) and micellar liquid chromatography using hybrid mobile phases containing sodium dodecyl sulfate (SDS) and propanol, butanol or pentanol as modifiers. Detection was performed with a variable wavelength UV-vis detector at 272 nm. After the application of an interpretative strategy for the selection of the optimimum mobile phase, caffeine and theophylline can be resolved and determined in serum samples by direct injection, using a mobile phase made up of 50 mM SDS-2.5% (v/v) propanol-10 mM KH2PO4, pH 7, with an analysis time below 5 min. Calibration was linear in the range 0.05 to 50 μg mL−1 with r > 0.999. The statistical evaluation of the method was examined by performing intra-day (n = 6) and inter-day calibration (n = 7) and was found to be satisfactory, with highly accurate and precise results. The proposed method was suitably validated and applied to the determination of caffeine and theophylline in serum samples of patients treated with bronchodilators.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1073, Issues 1–2, 6 May 2005, Pages 309-315
نویسندگان
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