کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9749189 | 1493803 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A rapid method of diagnosing pulmonary tuberculosis using stir bar sorptive extraction-thermal desorption-gas chromatography-mass spectrometry
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A fast method for detection of tuberculostearic acid (TBSA) in sputum samples is described. The samples, obtained from patients with known or suspected pulmonary tuberculosis, were decontaminated and concentrated before being analyzed by stir bar sorptive extraction-thermal desorption-gas chromatography-mass spectrometry (SBSE-TD-GC-MS). Prior to extraction, the mycobacterial lipids were hydrolyzed and then derivatized with ethyl chloroformate to increase the sorption of the compounds by the polydimethylsiloxane (PDMS) stir bar coating. The limit of detection (LOD) is 0.2 ng mlâ1. Four sputum samples that were classified by direct microscopy as smear-positive or negative were analyzed by GC-MS. TBSA was detected at concentrations ranging from 0.47 to 2.3 ng mlâ1. The method is sufficiently sensitive to detect TBSA directly in clinical samples without the need to culture the organisms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1071, Issues 1â2, 15 April 2005, Pages 135-139
Journal: Journal of Chromatography A - Volume 1071, Issues 1â2, 15 April 2005, Pages 135-139
نویسندگان
Adriana Stopforth, Andreas Tredoux, Andrew Crouch, Paul van Helden, Pat Sandra,