کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9753304 1494199 2005 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Separation and quantification of a novel two-component vaccine adjuvant
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Separation and quantification of a novel two-component vaccine adjuvant
چکیده انگلیسی
Two reversed-phase HPLC methods were developed for the quantitative determination of the two components of the novel vaccine adjuvant IC31. The adjuvant consists of a mixture of a synthetic oligodeoxynucleotide (ODN) and an 11-mer cationic peptide. The negatively charged oligodeoxynucleotide and the positively charged peptide form a complex that has to be quantitatively dissociated for analysis. Dissociation of the complex was achieved with a basic heparin solution (1000 IU/ml) when analyzing the ODN, whereas 30% acetic acid was used for the determination of the peptide. Both methods are suitable for identification and quantification but also for stability indicating investigations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 822, Issues 1–2, 5 August 2005, Pages 263-270
نویسندگان
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