کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9753344 | 1494201 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High-performance liquid chromatography measurement of hyperforin and its reduced derivatives in rodent plasma
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: High-performance liquid chromatography measurement of hyperforin and its reduced derivatives in rodent plasma High-performance liquid chromatography measurement of hyperforin and its reduced derivatives in rodent plasma](/preview/png/9753344.png)
چکیده انگلیسی
A reverse-phase high-performance liquid chromatography method was developed for the determination of hyperforin and its reduced derivatives octahydrohyperforin and tetrahydrohyperforin in rodent plasma. The procedure includes solid-phase extraction from plasma using the Baker 3cc C8 cartridge, resolution on the Symmetry Shield RP8 column (150 mm Ã 4.6 mm, i.d. 3.5 μm) and UV absorbance detection at 300 nm. The assay was linear over a wide range, with an overall coefficient of variation less than 10% for all compounds. The precision and accuracy were within acceptable limits and the limit of quantitation was sufficient for studies preliminarily assessing the disposition of tetrahydrohyperforin and octahydrohyperforin in the mouse and rat.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 816, Issues 1â2, 25 February 2005, Pages 21-27
Journal: Journal of Chromatography B - Volume 816, Issues 1â2, 25 February 2005, Pages 21-27
نویسندگان
M. Rozio, C. Fracasso, A. Riva, P. Morazzoni, S. Caccia,