کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9754445 | 1494678 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantification of propiverine by liquid chromatography/electrospray tandem mass spectrometry: Application to a bioequivalence study of two formulations in healthy subjects
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Quantification of propiverine by liquid chromatography/electrospray tandem mass spectrometry: Application to a bioequivalence study of two formulations in healthy subjects Quantification of propiverine by liquid chromatography/electrospray tandem mass spectrometry: Application to a bioequivalence study of two formulations in healthy subjects](/preview/png/9754445.png)
چکیده انگلیسی
Here we report on the development and validation of a sensitive and rapid reversed-phase liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantitative determination of propiverine in human plasma. After adding an internal standard (oxybutynin chlroride) to human plasma, samples were extracted using n-hexane/ethylacetate (8:2, v/v). Compounds extracted were analyzed by reversed-phase high-performance liquid chromatography (HPLC) with multiple reaction monitoring (MRM) mode for analyte detection. This method for determination of propiverine proved accurate and reproducible, with a limit of quantitation of 0.5Â ng/ml in human plasma. The standard calibration curve for propiverine was linear (r2Â =Â 0.9988) over the concentration range 0.5-1000.0Â ng/ml in human plasma. The intra- and inter-day precision over this concentration range was lower than 8.66% (relative standard deviation, %R.S.D.), and accuracy was between 99.46 and 109.41%, respectively. This method was successfully applied to a bioequivalence study of two propiverine hydrochloride tablet formulations (20Â mg) in 24 healthy subjects after a single administration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 39, Issues 3â4, 15 September 2005, Pages 670-676
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 39, Issues 3â4, 15 September 2005, Pages 670-676
نویسندگان
Sung-Hee Cho, Hun-Woo Lee, Ho-Taek Im, Wan-Su Park, Minsun Baek, Kyung-Tae Lee,