کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9754518 | 1494679 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A new method for the analysis of amikacin using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivatization and high-performance liquid chromatography with UV-detection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A simple and sensitive reversed-phase liquid chromatographic method has been developed for the determination of amikacin (AMK) by derivatization. The method is based on the pre-column derivatization of AMK with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC). The derivatization reaction proceeds in aqueous solution at room temperature with a borate buffer of pH 8.0. The formation of the corresponding derivative of AMK is instantaneous and it is stable for more than 36 h. Detection was performed by UV-absorption instead of fluorescence. Several factors influencing the derivatization reaction yields were studied and optimized. The system offered the following analytical parameters: limit of detection (LOD) of 0.068 μg mlâ1 (3Ï), linear correlation coefficient of 0.9998 and linear range response from 2 to 50 μg mlâ1. The precision of the method was <1%. As a preliminary application, the method has been successfully applied to the amikacin determination in parenteral pharmaceutical formulations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 39, Issues 1â2, 1 September 2005, Pages 294-298
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 39, Issues 1â2, 1 September 2005, Pages 294-298
نویسندگان
José F. Ovalles, MarÃa del Rosario Brunetto, Máximo Gallignani,